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rm colony-stimulating factor-1 (csf-1  (R&D Systems)


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    R&D Systems rm colony-stimulating factor-1 (csf-1
    Rm Colony Stimulating Factor 1 (Csf 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rm+colony-stimulating+factor-1+(csf-1/csf+1+protein/pm14978731-65-9-10
    Average 90 stars, based on 1 article reviews
    rm colony-stimulating factor-1 (csf-1 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: The Programmed Death-1 Pathway Counter-Regulates Inflammation-Induced Osteoclast Activity in Clinical and Experimental Settings
    Article Snippet: .. Cells were adjusted to a final concentration of 2 × 10 6 /ml and resuspended in RPMI with the addition of penicillin/streptomycin (5%) and recombinant mouse (rm) M-CSF (50 ng/ml) (R&D Systems, Abingdon, UK) and rmRANKL (25 ng/ml) (R&D Systems) for 7 days. .. At day 7, cultures were washed and stained for the presence of TRAP-positive cells (B-Bridge International, CA, USA).

    Recombinant:

    Article Title: The Programmed Death-1 Pathway Counter-Regulates Inflammation-Induced Osteoclast Activity in Clinical and Experimental Settings
    Article Snippet: .. Cells were adjusted to a final concentration of 2 × 10 6 /ml and resuspended in RPMI with the addition of penicillin/streptomycin (5%) and recombinant mouse (rm) M-CSF (50 ng/ml) (R&D Systems, Abingdon, UK) and rmRANKL (25 ng/ml) (R&D Systems) for 7 days. .. At day 7, cultures were washed and stained for the presence of TRAP-positive cells (B-Bridge International, CA, USA).

    Article Title: Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (1.077 g/ml; Invitrogen, Carlsbad, CA, USA) density gradient centrifugation. .. Monocytes were purified from PBMCs by using the Monocyte Negative Isolation Kits (Dynal, Biotech ASA, Oslo, Norway), and 1 × 10 6 cells/ml were cultured in 2 ml RPMI 1640 with 10% heat-inactivated fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) with recombinant human M-CSF (rM-CSF, 15 ng/ml; R&D Systems, Inc., Minneapolis, MN, USA) in 6-well plates at 37°C. ..

    Article Title: von Willebrand factor links primary hemostasis to innate immunity
    Article Snippet: Human plasma-derived von Willebrand factor-Factor VIII free (VWF) (Haematology Technologies Inc.), Recombinant von Willebrand factor, VonVendi® (r-VWF) (Takeda), Ultra pure lipopolysaccharide (LPS) (Sigma), INFγ (Life Technologies, Gibco), IL-4 (Life Technologies, Gibco), IL-10 (Life Technologies, Gibco), IL-13 (Life Technologies, Gibco). .. Recombinant mouse M-CSF (rm M-CSF) (R&D System), Cell Tracker green CMFDA Dye (Thermofisher), Seahorse XF Cell Mito Stress Cell Kit (Agilent), Primary antibodies human anti-p38, P-p38, JNK, P-JNK, IKBα, P-IKBα, p65, P-p65, HIF-1α, and β-actin (Cell Signaling Technologies), Alexa Fluor 488 conjugate (Molecular Probes, Thermofisher Scientific). .. Peripheral blood mononuclear cells (PBMC) were isolated from health donor buffy coats following histopaque (Sigma) gradient separation.

    Article Title: von Willebrand factor links primary hemostasis to innate immunity.
    Article Snippet: Human plasma-derived von Willebrand factor-Factor VIII free (VWF) (HaematologyTechnologies Inc.), Recombinant vonWillebrand factor, VonVendi® (r-VWF) (Takeda), Ultra pure lipopolysaccharide (LPS) (Sigma), INFγ (Life Technologies, Gibco), IL-4 (Life Technologies, Gibco), IL-10 (Life Technologies, Gibco), IL-13 (Life Technologies, Gibco). .. Recombinant mouse M-CSF (rm M-CSF) (R&D System), Cell Tracker green CMFDA Dye (Thermofisher), Seahorse XF Cell Mito Stress Cell Kit (Agilent), Primary antibodies human anti-p38, P-p38, JNK, P-JNK, IKBα, P-IKBα, p65, P-p65, HIF-1α, and β-actin (Cell Signaling Technologies), Alexa Fluor 488 conjugate (Molecular Probes, Thermofisher Scientific). .. Peripheral bloodmononuclear cells (PBMC) were isolated from health donor buffy coats following histopaque (Sigma) gradient separation.

    Article Title: IL-11 produced by breast cancer cells augments osteoclastogenesis by sustaining the pool of osteoclast progenitor cells
    Article Snippet: .. Recombinant mouse M-CSF (rM-CSF) (416-ML-010) and IL-11 (418-ML-005) were obtained from R&D Systems (Minneapolis, MN). .. Neutralizing anti-human IL-11 antibody (AB-218-NA) and normal goat IgG control antibody (AB-108-C) were also obtained from R&D Systems.

    Article Title: The 3.6 kb DNA fragment from the rat Col1a1 gene promoter drives the expression of genes in both osteoblast and osteoclast lineage cells.
    Article Snippet: The type I collagen promoter has been used to develop transgenic constructs that are able to mark different stages of osteoblastic differentiation.. The pOBCol3.6 promoter is active in early mesenchymal progenitors, including preosteoblasts and osteoblasts, while the pOBCol2.3 promoter is more restricted, showing expression in mature osteoblasts and osteocytes.. Transgenic mouse lines have been created that express various GFP reporters under the control of both promoters.

    Purification:

    Article Title: Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (1.077 g/ml; Invitrogen, Carlsbad, CA, USA) density gradient centrifugation. .. Monocytes were purified from PBMCs by using the Monocyte Negative Isolation Kits (Dynal, Biotech ASA, Oslo, Norway), and 1 × 10 6 cells/ml were cultured in 2 ml RPMI 1640 with 10% heat-inactivated fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) with recombinant human M-CSF (rM-CSF, 15 ng/ml; R&D Systems, Inc., Minneapolis, MN, USA) in 6-well plates at 37°C. ..

    Isolation:

    Article Title: Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (1.077 g/ml; Invitrogen, Carlsbad, CA, USA) density gradient centrifugation. .. Monocytes were purified from PBMCs by using the Monocyte Negative Isolation Kits (Dynal, Biotech ASA, Oslo, Norway), and 1 × 10 6 cells/ml were cultured in 2 ml RPMI 1640 with 10% heat-inactivated fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) with recombinant human M-CSF (rM-CSF, 15 ng/ml; R&D Systems, Inc., Minneapolis, MN, USA) in 6-well plates at 37°C. ..

    Cell Culture:

    Article Title: Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (1.077 g/ml; Invitrogen, Carlsbad, CA, USA) density gradient centrifugation. .. Monocytes were purified from PBMCs by using the Monocyte Negative Isolation Kits (Dynal, Biotech ASA, Oslo, Norway), and 1 × 10 6 cells/ml were cultured in 2 ml RPMI 1640 with 10% heat-inactivated fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) with recombinant human M-CSF (rM-CSF, 15 ng/ml; R&D Systems, Inc., Minneapolis, MN, USA) in 6-well plates at 37°C. ..

    other:

    Article Title: Mesenchymal stromal cells cross-present soluble exogenous antigens as part of their antigen-presenting cell properties.
    Article Snippet: Recent studies involving bone marrow mesenchymal stromal cells (MSCs) demonstrated that interferon (IFN)– stimulation induces major histocompatibility complex (MHC) class II–mediated antigen presentation in MSCs both in vitro and in vivo.. Concordantly, we investigated the ability of MSCs to present extracellular antigen through their MHC class I molecules, a process known as cross-presentation.. Using an in vitro antigen presentation assay, we demonstrated that murine MSCs can cross-present soluble ovalbumin (OVA) to naive CD8 T cells from OT-I mice.

    Article Title: Brucella abortus Invasion of Osteocytes Modulates Connexin 43 and Integrin Expression and Induces Osteoclastogenesis via Receptor Activator of NF-κB Ligand and Tumor Necrosis Factor Alpha Secretion
    Article Snippet: Briefly, bone marrow cells from BALB/c mice were cultured in complete medium containing 5 ng/ml murine recombinant M-CSF (rM-CSF) (R&D Systems, Minneapolis, MN, USA) for 12 h in 24-well plates.



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